Sivanadane Mandjiny

"Bioprocessing and Bioseparations"

As the Director of the Biotechnology Research and Training Center at COMTECH in Pembroke, I am deeply committed to advancing the field of Bioprocessing and Bioseparations. My current research focuses on biomanufacturing, particularly the bioprocessing of green fluorescent protein (GFP) as a model protein. This research encompasses both upstream and downstream processing. In upstream processing, we identify cell lines, cell stocking and fermentation to produce proteins. In downstream processing, we train on various unit operations such as centrifugation, homogenization, filtration and chromatographic methods.

My research also extends to the fermentation of other proteins and enzymes, with a keen interest in the characterization of tangential flow filtration and chromatography techniques. Ion exchange chromatography is a primary focus, as it has become the preferred method for purifying proteins and other macromolecules from complex biological fluids. Our research uses various solid matrices, such as membranes (including nylon and PVA) and gel beads (such as Sepharose and silica). We evaluate these matrices for their protein binding capacities, comparing membranes in filtration mode and gel beads in chromatographic columns. This comparative analysis provides valuable data on binding constants and adsorption capacities, which are crucial for optimizing downstream processing in the pharmaceutical industry.

Students working with me gain comprehensive training in basic lab techniques, literature research and oral and written communication. They also develop practical skills in the use of the Cytiva Chromatographic system. This hands-on approach ensures that students are well-prepared for careers in the pharmaceutical and biotechnological industries. By blending hands-on training with cutting-edge research, we aim to foster a deep understanding of bioprocessing and bioseparations. This prepares students for successful careers in the industry and contributes to the long-term development of new and efficient biomanufacturing processes. My commitment to student development is reflected in our ongoing efforts to train individuals with no prior background in biomanufacturing, helping them secure employment in the pharmaceutical industry.